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Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips
Author(s) -
Breadmore Michael C.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600463
Subject(s) - sensitivity (control systems) , electrochromatography , computer science , chromatography , biochemical engineering , capillary electrochromatography , nanotechnology , chemistry , capillary electrophoresis , materials science , engineering , electronic engineering
Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in‐line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10 000‐ to 100 000‐fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.