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MEKC: An update focusing on practical aspects
Author(s) -
Silva Manuel
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600455
Subject(s) - derivatization , chemistry , micelle , stacking , sensitivity (control systems) , resolution (logic) , chromatography , biochemical engineering , analytical chemistry (journal) , computer science , mass spectrometry , organic chemistry , artificial intelligence , electronic engineering , aqueous solution , engineering
This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on‐line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in‐capillary reactions and coupling with flow‐injection systems) and improvements in resolution obtained by changing the composition of the BGE ( e.g. , with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.