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A wide‐range, low‐cost 150 bp ladder for sizing DNA fragments between 150 and 4500 bp
Author(s) -
Barvish Zeev,
Davis Claytus,
Gitelman Inna
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600438
Subject(s) - agarose , agarose gel electrophoresis , plasmid , dna , gel electrophoresis , biology , microbiology and biotechnology , cloning (programming) , gel electrophoresis of nucleic acids , electrophoresis , sizing , computational biology , genome , chromatography , genetics , chemistry , computer science , gene , organic chemistry , programming language
Agarose gel electrophoresis, a very routine procedure, requires molecular weight standards; these are usually manufactured from plasmid or viral DNA fragments, or more recently, from PCR products of defined sizes. We describe here the preparation of a molecular weight standard from a completely different DNA source – the uniquely organized genome of the beetle Tenebrio molitor . The standard can be used to accurately size DNAs between 150 and 4500 bp, a useful range of sizes for many agarose gel electrophoresis applications, including separation of PCR products and plasmid cloning targets. In addition, it is easy to prepare, inexpensive, and rivals the best of the commercial ladders.