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Application of pressure cycling technology to tissue sample preparation for 2‐DE
Author(s) -
Ringham Heather,
Bell Richard L.,
Smejkal Gary B.,
Behnke James,
Witzmann Frank A.
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600434
Subject(s) - sample preparation , yield (engineering) , chromatography , posttranslational modification , chemistry , materials science , biological system , nanotechnology , computer science , biochemistry , biology , enzyme , metallurgy
2‐DE is a powerful protein analytical tool whose major strengths include semiglobal quantitation and charge separation of complex protein mixtures, enabling the analysis of differential protein expression, and variable post‐translational modification. One of 2‐DE's limitations relates to its limited dynamic range and consequently the number of proteins expressed that can be analyzed on a single gel. In an attempt to improve the yield of detectable proteins during sample preparation, we applied a novel extraction technique called pressure cycling technology.