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Separation of enantiomers of deprenyl with various CDs in CE and the effect of enantiomer migration order on enantiomeric impurity determination of selegiline in active ingredients and tablets
Author(s) -
CastroPuyana María,
Lomsadze Ketevan,
Crego Antonio L.,
Marina María Luisa,
Chankvetadze Bezhan
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600426
Subject(s) - enantiomer , selegiline , chemistry , impurity , chromatography , high performance liquid chromatography , enantiomeric excess , stereochemistry , organic chemistry , enantioselective synthesis , catalysis , medicine , disease , pathology , parkinson's disease
Opposite affinity pattern of enantiomers of the antiparkinsonian chiral drug deprenyl (DEP) was observed towards various neutral and charged derivatives of β‐CD. The effect of the enantiomer migration order on the LOD of enantiomeric impurity of R‐ DEP (selegiline) was studied for the standard substances and in the tablets from three different suppliers. The influence of injection mode on the LOD of a minor enantiomeric impurity was also studied and the CE method was compared with the pharmacopoeial HPLC method using a commercially available chiral column Chiralcel OD‐H. The optimized CE method was more suitable for low‐level enantiomeric impurity determination in selegiline compared to the pharmacopoeial HPLC method.