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Integrated isotachophoretic preconcentration with zone electrophoresis separation on a quartz microchip for UV detection of flavonoids
Author(s) -
Ma Bo,
Zhou Xiaomian,
Wang Gang,
Huang Huaiqing,
Dai Zhongpeng,
Qin Jianhua,
Lin Bingcheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600392
Subject(s) - chromatography , chemistry , capillary electrophoresis , detection limit , electrophoresis , isorhamnetin , analytical chemistry (journal) , microfluidics , quercetin , materials science , nanotechnology , kaempferol , biochemistry , antioxidant
A quartz microchip integrated isotachophoretic (ITP) preconcentration with zone electrophoresis (ZE) separation was fabricated using a novel multi‐point pressure method featured in normal temperature and lower pressure during bonding process. ITP followed by subsequential ZE of two flavonoids, quercetin and isorhamnetin on the microchip was performed consecutively on the homemade microfluidic workstation with UV detection, resulting in a decreased detectable concentration of 32‐fold, compared to the ZE mode only, and their detection limits decreased down to 0.2 µg/mL and 1.2 µg/mL, respectively