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A sheath‐flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis
Author(s) -
Mao Xiuli,
Chu Ivan K.,
Lin Bingcheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600349
Subject(s) - chemistry , chromatography , capillary electrophoresis , capillary action , microchannel , glycopeptide , micromanipulator , analytical chemistry (journal) , rnase p , ribonuclease , nanotechnology , materials science , biochemistry , rna , gene , antibiotics , artificial intelligence , computer science , composite material
Abstract Microchip was coupled with MS through a stable, sensitive, and controllable sheath‐flow nanoelectrospray (nES) interface for glycoprotein and glycopeptide analysis. The nano‐ESI (nESI) was made with a delivery capillary, a commercial nES capillary, and a stainless steel (SS) tube which were connected together through a tee unit. High voltage for nES was applied on the SS tube and the commercial nES capillary was used as nES emitter. The delivery capillary was attached to the microchannel for delivering liquid from microchip to the nESI source. The flow rate of sheath liquid was optimized to be 100–200 nL/min which largely reduced the sample dilution. The detection limit of peptides on this microchip/MS platform was at femtomole level. Glycoprotein and glycopeptides were also successfully analyzed on the platform. All the glycoforms and glycopeptides of ribonuclease B (RNase B) were identified with this method. Some structures of the glycopeptides from RNase B were further characterized with MS/MS on the microchip, coupled with a quadrupole IT‐MS.