Premium
Eigenmobilities in background electrolytes for CZE. V. Intensity (amplitudes) of system peaks
Author(s) -
Hruška Vlastimil,
Štědrý Milan,
Včeláková Kateřina,
Lokajová Jana,
Tesařová Eva,
Jaroš Michal,
Gaš Bohuslav
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600277
Subject(s) - analyte , conductivity , eigenvalues and eigenvectors , amplitude , matrix (chemical analysis) , signal (programming language) , intensity (physics) , analytical chemistry (journal) , electrolyte , biological system , chemistry , molar conductivity , electropherogram , computational physics , physics , optics , chromatography , electrode , computer science , ion , capillary electrophoresis , organic chemistry , quantum mechanics , biology , programming language
We present a mathematical model of CZE based on the concept of eigenmobilities - the eigenvalues of matrix M tied to the linearized governing equations of electromigration, and the spectral decomposition of matrix M into matrices of amplitudes P(j). Any peak in an electropherogram, regardless of whether it is an analyte peak or a system peak (system zone), is matched with its matrix P(j). This enables calculation of the peak parameters, such as the transfer ratio and the molar conductivity detection response (which give the indirect detection signal and the conductivity detection signal, respectively), when the initial disturbance caused by the injection of the sample is known. We also introduce new quantities, such as the generalized transfer ratio and the conductivity response of system zones, and show how the amplitude (intensity, area) of the analyte peaks and the system peaks can be calculated. We offer a free software, PeakMaster (http://www.natur.cuni.cz/gas), which yields this information in a user-friendly way.