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CE to monitor endotoxins by protein complexation
Author(s) -
Kilár Anikó,
Kocsis Béla,
Kustos Ildikó,
Kilár Ferenc,
Hjertén Stellan
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600276
Subject(s) - electrophoresis , salmonella , chemistry , hemoglobin , shigella sonnei , microbiology and biotechnology , bacteria , chromatography , shigella , biochemistry , biology , genetics
A new CE method for fast and efficient analysis of bacterial endotoxins (lipopolysaccharides) is described. It is based on the strong interaction between proteins and endotoxins. The UV absorption of the protein component in the complex is used for the detection. The electrophoretic mobility of the complex hemoglobin/endotoxin can be employed for qualitative analysis of the endotoxin. For instance, the structural differences between “smooth” and “rough” lipopolysaccharides from Salmonella minnesota (wild‐type), Salmonella minnesota R595 and Shigella sonnei R562H are reflected in the electrophoretic mobilities of their hemoglobin complex.