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Analysis of triazines by capillary electrochromatography/electrospray ionization‐mass spectrometry using a low‐flow sheath liquid interface
Author(s) -
Chang ChiaHao,
Chen ChaoJung,
Chuang YuChieh,
Her GuorRong
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600251
Subject(s) - capillary electrochromatography , chemistry , chromatography , electrospray , electrospray ionization , buffer (optical fiber) , elution , analytical chemistry (journal) , mass spectrometry , volumetric flow rate , gradient elution , triazine , pulmonary surfactant , capillary electrophoresis–mass spectrometry , buffer solution , capillary action , electrochromatography , high performance liquid chromatography , materials science , capillary electrophoresis , telecommunications , physics , quantum mechanics , computer science , composite material , biochemistry , polymer chemistry
CEC‐MS has been used for the analysis of eight‐triazine herbicides. It showed significantly better S/N ratio than reversed EOF CE‐MS and MEKC‐MS, due to the lack of a surfactant in the separation buffer. By optimizing the pH, the organic content of the running buffer, and the separation potential, optimal separation was achieved within 18 min using a running buffer of pH 7.0, containing 70% v/v ACN, and an applied voltage of 17 kV. Gradient CEC showed superior separation when compared with isocratic elution. The combination of a tapered CEC column and a low‐flow interface confers several advantages including better sensitivity, low dead volume, and independent control of the conditions used for CEC separation and ESI analysis.