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Transient isotachophoresis in carrier ampholyte‐based capillary electrophoresis for protein analysis
Author(s) -
Busnel JeanMarc,
Descroix Stéphanie,
Godfrin Dominique,
Hennion MarieClaire,
Kašička Václav,
Peltre Gabriel
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600230
Subject(s) - isotachophoresis , capillary electrophoresis , chromatography , transient (computer programming) , chemistry , electrophoresis , electrolyte , computer science , electrode , operating system
Transient ITP (t‐ITP) has been used in carrier ampholyte‐based CE (CABCE) to enhance the sensitivity of protein analysis. The characteristics of carrier ampholytes (CAs) narrow pH cuts‐based buffers, when used as BGEs in CE are compatible with t‐ITP requirements. Indeed, being the sole buffering species of such solutions, CAs impose a pH close to their p I . Thus, in these solutions, the CAs possess low electrophoretic mobility. As a consequence, by adding an ionic component with high electrophoretic mobility either in the studied sample or in the BGE, a t‐ITP step can be generated. This has first been demonstrated for protein test mixtures. Then, the combination of t‐ITP with CABCE has been applied to study a real sample, the bovine milk.