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A self‐contained polymeric 2‐DE chip system for rapid and easy analysis
Author(s) -
Usui Keisuke,
Hiratsuka Atsunori,
Shiseki Kisho,
Maruo Yuji,
Matsushima Toshiyuki,
Takahashi Katsuyoshi,
Unuma Yutaka,
Sakairi Koji,
Namatame Ichiji,
Ogawa Yasuhiro,
Yokoyama Kenji
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600221
Subject(s) - chip , port (circuit theory) , sample (material) , materials science , leakage (economics) , lab on a chip , computer science , chromatography , computer hardware , analytical chemistry (journal) , nanotechnology , microfluidics , chemistry , electrical engineering , engineering , telecommunications , economics , macroeconomics
Abstract We developed a polymeric 2‐DE chip system. The chip consisted of an IEF region, an SDS‐PAGE region, a valveless connection port, and a sample introduction port. A “junction structure” as a valveless connection port, which allowed separating and connecting the first‐ and second‐dimensional gels, was fabricated between their regions. A “solution inlet” as a sample introduction port was fabricated to perform the liquid and sample introductions without solution leakage. Simultaneous sample monitoring was performed using the on‐chip detection system. The performances of the system were demonstrated using commercially available proteins as a standard specimen and tissue‐extracted proteins as the real samples. All procedures were employed without any movement of relocation part. This new 2‐D separation system realized improved labor‐intensive operations and a reduced experimental time.