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A fast and reliable route integrating calibration and analysis protocols for water‐soluble vitamin determination on microchip‐electrochemistry platforms
Author(s) -
Crevillén Agustín González,
Blasco Antonio Javier,
González María Cristina,
Escarpa Alberto
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600213
Subject(s) - analyte , calibration , reproducibility , folic acid , chromatography , calibration curve , chemistry , protocol (science) , electrochemistry , analytical chemistry (journal) , computer science , electrode , detection limit , mathematics , medicine , statistics , alternative medicine , pathology
Abstract A novel analytical route to determine water‐soluble vitamins (B group and C) using single channel microchip‐electrochemistry platforms is presented. The electrochemical detection protocol was carefully optimized, and it was shown that it was crucial to use 1 M nitric acid in the detector compartment to detect folic acid. A phosphate buffer (pH 6, 10 mM) and a separation voltage of 2 kV gave the complete separation of vitamins in less than 130 s, with good reproducibility (RSDs less than 10%) and accuracy (error less than 9%). In addition, a methodological innovation integrating calibration and analysis of water‐soluble vitamins on the chip is also proposed. The strategy consisted in sequentially using both reservoirs (named calibration and analysis reservoirs) as well as a calibration factor (defined as signal/concentration of analyte). The analytical route required 350 s in the overall protocol (employing 130 s in calibration plus 130 s in analysis), an improvement over the times used in both conventional and microchip protocols.