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Analysis of selected constituents in methanolic extracts of Hypericum perforatum collected in different localities by capillary ITP‐CZE
Author(s) -
Hamoudová Rafífa,
Pospíšilová Marie,
Spilková Jiřina
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600193
Subject(s) - quercitrin , chemistry , chromatography , hypericum perforatum , chlorogenic acid , rutin , capillary electrophoresis , boric acid , kaempferol , hyperforin , hypericum , quercetin , quinic acid , tris , traditional medicine , biochemistry , organic chemistry , medicine , antioxidant
The on‐line combination of CZE with capillary ITP (ITP‐CZE) was used for the separation and quantification of selected flavonoids and phenolic acids in Hypericum perforatum leaves and flowers collected in six different localities in Slovakia. The leading electrolyte in the ITP preseparation step was 10 mM HCl with Tris as counterion (pH* 7.2). The terminating electrolyte was 50 mM boric acid of pH* 8.2 (adjusted with barium hydroxide). The BGE in the electrophoretic step contained 25 mM β‐hydroxy‐4‐morpholinopropanesulfonic acid (MOPSO), 50 mM Tris, 65 mM boric acid, pH* 8.3. The content of methanol in all electrolytes was 20% v/v. The total time of the analysis (including the preseparation step) was ∼35 min. The rectilinear calibration ranges were between 0.125 and 5.0 µg/mL with kaempferol as internal standard. The correlation coefficients ranged between 0.9912 (for quercitrin and chlorogenic acid) and 0.9988 (for isoquercitrin). The RSD values are between 0.86 and 7.78% ( n = 6) when determining rutin and quercetin (4 µg/mL). The optimized method was employed for the assay of flavonoids in medicinal plant extract of different collections of Hypericum perforatum haulm. The variability of the content of the active components depending on the place of collection was confirmed.