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Application of capillary zone electrophoresis with large‐volume sample stacking to the sensitive determination of sulfonamides in meat and ground water
Author(s) -
SotoChinchilla Jorge J.,
GarcíaCampaña Ana M.,
GámizGracia Laura,
CrucesBlanco Carmen
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600166
Subject(s) - sulfamerazine , sulfadimethoxine , chromatography , chemistry , capillary electrophoresis , sulfapyridine , sulfadiazine , stacking , extraction (chemistry) , methanol , solvent , detection limit , analytical chemistry (journal) , biochemistry , organic chemistry , antibiotics
A CZE method with UV‐Vis detection has been established and validated for the determination of nine sulfonamides: sulfapyridine, sulfamethazine, sulfamerazine, sulfamether, sulfadiazine, sulfadimethoxine, sulfamethoxazole, sulfachlorpyridazine, and sulfamethizole. Optimum separation was obtained on a 64.5 cm×75 μm bubble cell capillary using a buffer containing 45 mM sodium phosphate and 10% methanol at pH 7.3, with temperature and voltage of 27°C and 25 kV, respectively. p ‐Aminobenzoic acid was used as an internal standard . Taking into account the lack of sensitivity of the UV‐Vis detection, the application of an on‐line preconcentration methodology, such as large‐volume sample stacking with polarity switching has been proposed. This procedure combined with a solvent extraction/SPE method applied for off‐line preconcentration and cleanup provides a significant improvement in the LODs, ranging from 2.59 to 22.95 μg/L for the studied compounds; the quantification of these residues being possible below the levels established by EU legislation in animal food products, such as meat. Satisfactory recoveries were also obtained in the analysis of these compounds in ground water.