Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment

A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H 3 BO 3 in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused‐silica capillary of 50 µm id×375 µm od×54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1–100 µg/mL. The detection limits were less than 0.70 µg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N ‐( O,O‐ diisopropyl) phosphoryl amino acid and adenosine.