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Development of a NACE method for simultaneous measurement of three adenosine monophosphate isomers in biomimicking prebiotic synthesis without sample pretreatment
Author(s) -
Song Juanmei,
Liu Hongxia,
Han Po,
Zhao Canfang,
Wu Yangjie,
Zhang Shusheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600164
Subject(s) - chemistry , adenosine , chromatography , detection limit , calibration curve , prebiotic , tris , analytical chemistry (journal) , linear range , food science , biochemistry
A practical NACE method was developed for simultaneous determination of three adenosine monophosphate (AMP) isomers. Separation of three AMP isomers was achieved using 200 mM Tris/H 3 BO 3 in acetontrile/water (2:1 v/v) at pH* 10.0 as the running buffer and +25 kV as the applied voltage over a bare fused‐silica capillary of 50 µm id×375 µm od×54.5 cm (46 cm to the detector window). At 260 nm, the calibration curves were linear in the range of 1–100 µg/mL. The detection limits were less than 0.70 µg/mL. The recovery ranged from 94.5 to 106.4%. The intraday RSDs of the migration times were between 2.1 and 3.0%. The developed NACE method has been successfully applied for the determination of three AMP isomers in the real samples of biomimicking prebiotic synthesis reaction between N ‐( O,O‐ diisopropyl) phosphoryl amino acid and adenosine.