Premium
Electrochromatographic behavior of silica monolithic capillaries of different skeleton sizes synthesized with a simplified and shortened sol–gel procedure
Author(s) -
Puy Guillaume,
Demesmay Claire,
Rocca JeanLouis,
Iapichella Julien,
Galarneau Anne,
Brunel Daniel
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600153
Subject(s) - calcination , monolith , materials science , electrochromatography , capillary electrochromatography , skeleton (computer programming) , chromatography , chemical engineering , ionic strength , chemistry , composite material , computer science , capillary action , organic chemistry , catalysis , aqueous solution , engineering , programming language
Silica monolithic capillaries (SMCs) were synthesized by a sol–gel process. First, a simplification of the synthesis was proposed by replacing the calcination and the drying steps which can have tremendous effects on chromatographic and physical properties, by a single water or methanol 2 h washing step. The efficiency of such a washing step was demonstrated and the comparison of the chromatographic and electrochromatographic properties between calcined and washed SMCs has shown that such a modification did not impair retention, efficiency, and stability of the monolith. This simplified procedure was carried out to synthesize SMCs with two different skeleton sizes. These capillaries were evaluated in electrochromatography and present high efficiencies ( H = 5 µm) at least equal to the best ones reported in the literature. Furthermore, the influence of the skeleton size on the EOF of the second kind (EOF‐2) was investigated with unmodified SMCs used under various experimental conditions including electrical field strength and buffer concentration. The ionic strength of the mobile phase and the applied electrical field that enable this EOF‐2 were related to the size of the skeleton which was tuned by the synthesis conditions.