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Fabrication and characterization of semicircular detection electrodes for contactless conductivity detector – CE microchips
Author(s) -
Lee ChiaYen,
Chen C. M.,
Chang GuanLiang,
Lin CheHsin,
Fu LungMing
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600113
Subject(s) - microfabrication , materials science , biochip , microscale chemistry , electrode , microfluidics , detector , optoelectronics , fabrication , polydimethylsiloxane , nanotechnology , chemistry , computer science , medicine , alternative medicine , mathematics education , mathematics , pathology , telecommunications
This study uses simple and reliable microfabrication techniques to fabricate CE biochips, integrating a novel contactless conductivity detector in a miniaturized detection system in a microfluidic biochip. The off‐channel electrodes are deposited around side channels by Au sputtering and patterned using a standard “lift‐off” process. A vacuum fusion bonding process is employed to seal the lower substrate containing the microchannels and the electrodes to an upper glass cover plate. The variations in the capacitance between the semicircular detection electrodes in the side channels are measured as different samples and ions pass through the detection region of the CE separation channel. Samples of Rhodamine B, commercial sports drinks, mineral waters, and a red wine, respectively, are mixed in different buffer solutions, separated, and successfully detected using the developed device. The semicircular detection electrodes for the contactless conductivity detector have microscale dimensions and provide a valuable contribution to the realization of the lab‐on‐a‐chip concept.