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Numerical model for DNA loading in microdevices: Stacking and autogating effects
Author(s) -
ElDifrawy Sameh A.,
Srivastava Alok,
Gismondi Elizabeth A.,
McKenna Brian K.,
Ehrlich Daniel J.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600088
Subject(s) - stacking , materials science , dna , mechanics , chemistry , physics , organic chemistry , biochemistry
Abstract Many electrophoresis‐based DNA sequencing and genotyping microdevices rely on field‐driven effects to load and preconcentrate the sample. A quantitative model is developed for a broad class of electrophoresis‐based microfabricated sample injectors. Quantitative predictions of DNA preconcentration are compared with experimental data and are shown to qualitatively reproduce the detailed time‐evolving sample distribution in the injector. The model provides practical guidance on device and protocol design, in order to optimize this critical aspect of microfluidic devices.