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Permanent surface modification of polymeric capillary electrophoresis microchips for protein and peptide analysis
Author(s) -
Liu Jikun,
Lee Milton L.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600082
Subject(s) - capillary electrophoresis , electrophoresis , peptide , chromatography , capillary action , surface modification , chemistry , capillary surface , capillary electrochromatography , biophysics , materials science , biochemistry , biology , composite material
Because of their surface heterogeneity, proteins readily adsorb on polymeric substrates via various interactions, which adversely affects the performance of polymeric microfluidic devices in electrophoresis‐based protein/peptide analysis. Therefore, it is necessary to use surface modification techniques such as dynamic coating or more complicated permanent surface modification, which has broader application and better performance, to render the polymeric microchannels protein‐resistant. This manuscript is a review of the surface chemistry of microfluidic devices used for electrophoretic separations of proteins and peptides. The structural complexity of proteins as it relates to adsorption is described, followed by a review of the mechanisms and structural characteristics of protein‐resistant surfaces. Permanent surface modification techniques used in grafting protein‐resistant materials onto the surfaces of electrophoresis microchannels fabricated from polymer substrates are summarized and successful examples are presented.