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Metabolite profiling of human urine by CE‐ESI‐MS using separation electrolytes at low pH
Author(s) -
Benavente Fernando,
van der Heijden Rob,
Tjaden Ubbo R.,
van der Greef Jan,
Hankemeier Thomas
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600055
Subject(s) - chemistry , formic acid , chromatography , metabolite , electrospray , acetic acid , electrospray ionization , urine , capillary electrophoresis–mass spectrometry , electrolyte , mass spectrometry , organic chemistry , biochemistry , electrode
We investigated the potential of CE coupled to electrospray MS (CE‐ESI‐MS) in metabolite profiling of human urine without any sample prefractionation step. A heterogeneous mixture of biologically relevant compounds covering a broad range of physicochemical properties was used to optimize separation conditions in fused‐silica capillaries. A running electrolyte containing 50 mM of acetic acid and 50 mM of formic acid at pH 2.5 was used for the CE separations. A sheath‐flow electrospray interface was employed for CE‐ESI‐MS analysis. Sheath liquids containing 80:20 v/v methanol/water with 0.1% v/v of acetic acid or 60:40 v/v isopropanol/water with 0.5% v/v of ammonia were selected for optimum detection in positive and negative ESI modes, respectively. Reproducibility and sensitivity were studied, and strategies for identification of the separated urinary compounds are suggested. We report major advantages and disadvantages of CE‐ESI‐MS for metabolite profiling of human body fluids. This work may be regarded as a first step in the use of CE‐ESI‐MS for reliable differential analysis of body fluids from healthy and diseased individuals.