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Separation of doxorubicin and doxorubicinol by cyclodextrin‐modified micellar electrokinetic capillary chromatography
Author(s) -
Eder Angela R.,
Chen Jennifer S.,
Arriaga Edgar A.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200600025
Subject(s) - micellar electrokinetic chromatography , chromatography , capillary electrophoresis , doxorubicin , chemistry , detection limit , metabolite , cyclodextrin , enantiomer , electrokinetic phenomena , chemotherapy , biochemistry , stereochemistry , biology , genetics
Doxorubicinol (DOXol) is a human metabolite of the chemotherapy agent doxorubicin (DOX), and is associated with dose‐dependent cardiotoxicity and decreased drug efficacy. Due to the structural similarities and equal molecular charges of DOXol and DOX, their electrophoretic separation is commonly ineffective. A method for separating and detecting DOX and DOXol, as well as two DOXol enantiomers, was established using cyclodextrin‐modified micellar electrokinetic capillary chromatography with laser‐induced fluorescence detection. Differential DOXol production was detected in a DOX‐sensitive and resistant pair of cell lines, with a 0.08 ± 0.01 fmol limit of detection.