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Electrophoresis of pharmaceutical proteins: Status quo
Author(s) -
Little Michael J.,
Paquette Donald M.,
Roos Pieter K.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500951
Subject(s) - capillary electrophoresis , biopharmaceutical , gel electrophoresis of proteins , gel electrophoresis , status quo , chemistry , computational biology , chromatography , polyacrylamide gel electrophoresis , microbiology and biotechnology , biology , biochemistry , political science , law , enzyme
The biotechnology industry has undergone rapid growth in recent years largely due to the development and success of protein‐based therapeutics for a wide range of disorders. Similar to traditional pharmaceuticals, characterization of a therapeutic protein for its physicochemical properties, process monitoring and lot release is crucial. Electrophoresis in the slab‐gel format has and continues to be a mainstay of the protein laboratory; and more recently, CE has begun to make significant inroads for protein analysis in industrial settings. This review focuses on the electrophoresis of proteins with an emphasis on protein‐based therapeutics in the capillary, slab‐gel and to a lesser extent, the microchip format. Reported applications of electrophoresis at several stages of the biopharmaceutical industry covering the period of 2000–2005 will be discussed.