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Capillary electrophoresis‐mass spectrometry of citrus endophytic bacteria siderophores
Author(s) -
Simionato Ana V. Colnaghi,
Simó Carolina,
Cifuentes Alejandro,
Lacava Paulo Teixeira,
Araújo Wellington Luiz,
Azevedo João Lúcio,
Carrilho Emanuel
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500933
Subject(s) - siderophore , methylobacterium , formic acid , chromatography , chemistry , mass spectrometry , bacteria , biology , biochemistry , genetics , 16s ribosomal rna , gene
CE‐ESI‐MS with a liquid sheath interface and IT mass analyzer was used for analysis of siderophores from different strains of Methylobacterium spp. citrus endophyte extracts. Three Methylobacterium strains were investigated according to positive bioassay tests. Bacteria cultures were grown under Fe(III) absence (siderophore producing cultures) and under Fe(III) presence (control cultures). Siderophores were extracted from culture supernatant with polystyrene resins. BGE and sheath‐liquid composition were optimized, respectively, in order to assure both, best peak resolution and ESI‐MS sensitivity. The best analysis conditions were obtained with 100 mmol/L ammonium bicarbonate at pH 8 as BGE and methanol:H 2 O 25:75 + 0.05% formic acid as sheath liquid. CZE‐ESI‐MS analysis revealed two possible siderophores, according to bacterium species, presenting M r of 1004.3 and 798.3 Da.