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Studying protein‐drug interaction by microfluidic chip affinity capillary electrophoresis with indirect laser‐induced fluorescence detection
Author(s) -
Liu Xiaojun,
Liu Xin,
Liang Aiye,
Shen Zheng,
Zhang Yu,
Dai Zhongpeng,
Xiong Bohui,
Lin Bingcheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500890
Subject(s) - capillary electrophoresis , microfluidics , electrophoresis , fluorescence , chromatography , microfluidic chip , laser induced fluorescence , chemistry , fluorescein , heparin , analytical chemistry (journal) , materials science , nanotechnology , biochemistry , physics , quantum mechanics
Abstract We developed a microfluidic chip‐affinity CE method based on indirect LIF detection to study protein‐drug interactions. The interaction between heparin and BSA was quantitatively studied, as a model system. In our method, sodium fluorescein was chosen as background, and redistilled water as marker to monitor EOF. The electrophoretic mobility changes of BSA were measured, with various concentrations of heparin added to the running buffer. Each run was completed within 80 s. The binding constant was determined to be (1.24 ± 0.05)×10 3 M −1 , which was in good agreement with that reported in the literature.