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Peak identification in capillary isoelectric focusing using the concept of relative peak position as determined by two isoelectric point markers
Author(s) -
Wu Jiaqi,
Huang Tiemin
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500889
Subject(s) - isoelectric focusing , chemistry , isoelectric point , analytical chemistry (journal) , chromatography , sample (material) , pi , position (finance) , linearity , capillary action , physics , thermodynamics , biochemistry , finance , quantum mechanics , economics , enzyme
In CIEF analysis, sample peaks can be identified by their relative peak positions (RPP) that are determined using only two internal p I markers. The two internal p I marker peaks should bracket, as close as possible, the sample peaks. The RPP values of the sample peaks are then calculated using the p I values, peak positions of the two p I markers, and peak position of the sample. Use of this method can effectively compensate for pH gradient distortions that often occur as a result of salts. Also, as shown by the results of this paper, regardless of the linearity of the pH gradient established by the given carrier ampholytes, sample peaks can be identified within an SD of 0.1 pH unit in RPP (<2% RSD) as long as the sample is run using the same carrier ampholytes and maintaining salt concentrations in the range of 0–15 mM.