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Analysis of amphetamine, methamphetamine and methylenedioxy‐methamphetamine by micellar capillary electrophoresis using cation‐selective exhaustive injection
Author(s) -
Meng Pinjia,
Fang Ning,
Wang Meng,
Liu Huwei,
Chen David D.Y.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500886
Subject(s) - micellar electrokinetic chromatography , chemistry , chromatography , capillary electrophoresis , methylenedioxy , sodium dodecyl sulfate , methamphetamine , amphetamine , detection limit , electrolyte , pharmacology , organic chemistry , medicine , alkyl , halogen , electrode , neuroscience , dopamine , biology
Cation‐selective exhaustive injection (CSEI) is used as an on‐line concentration method for the high‐sensitivity analysis of illicit amphetamines using CE. Optimum conditions for the determination of amphetamine, methamphetamine and methylenedioxy‐methamphetamine were investigated. Sodium dodecyl sulfate (25 mM) in 100 mM phosphate buffer (pH 2.9) with 20% methanol as organic additive was used as the background electrolyte for CE separation. The LOD, based on an S/N of 3:1, was about 0.01 μg/mL using normal capillary micellar electrokinetic chromatography, while by using CSEI in combination with micellar sweeping the sensitivity increased up to 1000‐fold with the LOD lower than 50 pg/mL. The reproducibility of CSEI combined with micellar sweeping for analyzing amphetamines was satisfactory (relative standard deviation around 10% by using area ratios against an internal standard). This method is highly sensitive and can be used to analyze trace amount amphetamines in human hair.