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Immobilization of phospholipid‐avidin on fused‐silica capillaries for chiral separation in open‐tubular capillary electrochromatography
Author(s) -
Han NanYin,
Hautala Jari T.,
Bo Tao,
Wiedmer Susanne K.,
Riekkola MarjaLiisa
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500884
Subject(s) - capillary electrochromatography , phospholipid , chromatography , chemistry , capillary action , electrochromatography , avidin , capillary electrophoresis , materials science , membrane , biotin , biochemistry , composite material
Phospholipid‐coated fused‐silica capillaries with immobilized avidin were applied in the chiral separation of D,L ‐tryptophan, D,L ‐PTH‐serine, and D,L ‐PTH‐threonine at pH 7.4 by open‐tubular CEC. Liposomes prepared from 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphocholine, 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphoethanolamine‐ N ‐(Cap biotinyl), or 1,2‐dipalmitoyl‐ sn ‐glycero‐3‐phosphoethanolamine‐ N ‐(Biotinyl) with different amounts of phosphatidylserine were assessed as phospholipid coating materials. The stability of the coating and the success of the coating procedure were evaluated in terms of the repeatability of the enantiomer migration times and the resolution of enantiomers. The coating procedure itself significantly affected the migration times and resolution of the enantiomers. Reliable chiral separations with high separation efficiencies were achieved through careful choice of the coating method.