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CE as orthogonal technique to HPLC for alprazolam degradation product identification
Author(s) -
Saavedra Luis,
Huidobro Angel Luis,
García Antonia,
Cabanelas Jose Carlos,
González María G.,
Barbas Coral
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500882
Subject(s) - degradation (telecommunications) , chromatography , chemistry , high performance liquid chromatography , capillary action , analyte , forced degradation , adsorption , amine gas treating , alprazolam , polymer , reversed phase chromatography , organic chemistry , materials science , telecommunications , computer science , composite material , psychology , anxiety , psychiatry
The control of degradation products is currently a critical issue to the pharmaceutical industry. A degradation product that appeared in alprazolam tablets during their stability assay, 7‐chloro‐1‐methyl‐5‐phenyl‐[1,2,4]triazolo[4,3‐a]quinolin‐4‐amine, also named triazolaminoquinoline, was tested as possible candidate in the HPLC method employed for the study. The impurity showed the same retention time and spectra as the degradation product; but as all these compounds are very closely related, a confirmation with an independent technique was necessary, and CE was chosen for that purpose. Problems related to the adsorption of the analytes to the negatively charged silica surface were solved by employing a new polymeric capillary coating consisting of poly(3‐aminopropylmethylsiloxane). The polymer provided EOF towards the anode, and the two compounds were separated in less than 8 min in a 60 cm total‐length capillary, 75 µm id capillary with a BGE containing 50 mM phosphate buffer at pH 2.0 with 20% ACN. When the sample containing the degradation product was injected, the presence of triazolaminoquinoline was confirmed.