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Determination of quinolone residues in chicken and fish by capillary electrophoresis‐mass spectrometry
Author(s) -
JuanGarcía Ana,
Font Guillermina,
Picó Yolanda
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500868
Subject(s) - flumequine , chemistry , danofloxacin , chromatography , capillary electrophoresis , enrofloxacin , quinolone , mass spectrometry , antibiotics , biochemistry , ciprofloxacin
A specific pressure‐assisted CE‐MS method is described for the analysis of five quinolone residues. MS using a single quadrupole is compared with multiple‐stage MS using a quadrupole IT (QIT‐MS n ). The procedure involves a common sample preparation by SPE on disposable cartridges. The most suitable electrolyte is 60 mM (NH 4 ) 2 CO 3 at pH 9.2. Single quadrupole does not provide enough fragmentation to confirm identities according to the current legislation. However, QIT‐MS n achieves selective fragmentation. Using this method, danofloxacin, enrofloxacin, flumequine, ofloxacin, and pipemidic acid are analyzed in fortified samples of chicken and fish. Recoveries at levels of 50 ng/g were 62–99%, except for flumequine, which gives recoveries ≥45%. RSDs are from 9 to 16% and the LOD is equal (20 ng/g) for the five analytes. Confirmation of the quinolones' identity is achieved using QIT‐MS 3 . Forty samples of chicken and fish taken from different local markets are analyzed. Enrofloxacin is also determined in incurred chicken muscle using this method.