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Sensitive determination of carbohydrates labelled with p ‐nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light‐emitting diode
Author(s) -
Momenbeik Fariborz,
Johns Cameron,
Breadmore Michael C.,
Hilder Emily F.,
Macka Miroslav,
Haddad Paul R.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500856
Subject(s) - detection limit , chemistry , absorbance , chromatography , molar absorptivity , reagent , boric acid , capillary electrophoresis , analytical chemistry (journal) , yield (engineering) , materials science , physics , organic chemistry , optics , metallurgy
p ‐Nitroaniline was explored as a derivatising reagent for UV absorbance detection of carbohydrates after separation by CE. This derivatising agent has three advantages: first, it has excellent water solubility; second, it has high molar absorptivity; and third, it is possible to obtain sensitive detection using a UV or blue light‐emitting diode (LED) as the light source. The labelling reaction took less than 30 min to complete with high reaction yield. The separation process was modelled and optimised using an artificial neural network. Nine carbohydrates were separated by a CE system within 16 min using a 0.17 M boric acid buffer at pH 9.7. On‐column LED detection at 406 nm allowed the detection of carbohydrates with good detection limits (<1.1 µM or 8.8 fmol) and reproducible quantification in the concentration range of 2.6–200 µM. This method was applied successfully to the determination of component carbohydrates in some food samples.