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Capillary electrophoresis for the analysis of glycoprotein pharmaceuticals
Author(s) -
Kamoda Satoru,
Kakehi Kazuaki
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500853
Subject(s) - glycoprotein , glycan , oligosaccharide , chemistry , capillary electrophoresis , glycosylation , biochemistry , glycosidic bond , computational biology , chromatography , biology , enzyme
Carbohydrate chains in glycoprotein pharmaceuticals play important roles for the expression of their biological activities, but the structure and compositions of carbohydrate chains are dependent on the conditions for their production. Therefore, evaluation of the carbohydrate chains is quite important for productive process development, characterization of product for approval application, and routine quality control. The oligosaccharides themselves have complex structure including blanching and various glycosidic linkages, and oligosaccharides in one glycoprotein pharmaceutical generally have high heterogeneity, and characterization of oligosaccharide moiety in glycoprotein has been a challenging target. In these situations, CE has been realized as a powerful tool for oligosaccharide analysis due to its high resolution and automatic operating system. This review focuses on the application of CE to the glycoform analysis of glycoproteins and profiling of the N‐ linked glycans released from glycoprotein pharmaceuticals. Current applications for structure analysis using CE‐MS n technique and glycan profiling method for therapeutic antibody are also described.