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Nanostructured polymer matrix for oligonucleotide separation
Author(s) -
Zhang Jun,
Burger Christian,
Chu Benjamin
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500839
Subject(s) - oligonucleotide , matrix (chemical analysis) , polymer , separation method , materials science , separation (statistics) , nanotechnology , chemistry , chromatography , dna , computer science , composite material , biochemistry , machine learning
A nanostructured copolymer matrix has successfully separated oligonucleotides with high resolution by CE using a very short separation channel which simulates the real microchip condition for the first time. The triblock copolymer, E 45 B 14 E 45 (B20‐5000) with E, B, and subscript denoting oxyethylene, oxybutylene, and segment length, respectively, has a unique temperature‐dependent viscosity‐adjustable property and a dynamic coating ability in 1×TBE buffer (89 mM Tris, 89 mM boric acid, 2 mM EDTA in Milli‐Q water). The B‐block is hydrophilic at low temperatures, e.g. , 4°C, and the polymer solution has a very low viscosity of about 100 cP in a 32.5% w/v solution. At room temperatures, the B‐block becomes hydrophobic due to a breakdown of hydrogen bonds between B‐blocks and water, and the polymer matrix forms a body‐centered cubic structure at high concentrations. Oligonucleotide sizing markers ranging from 8 to 32 base could be successfully separated with one‐base resolution in a 1.5 cm long separation channel by E 45 B 14 E 45 in its gel‐like state.