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Sheathless capillary electrophoresis‐mass spectrometry using a pulsed electrospray ionization source
Author(s) -
Chao BingFong,
Chen ChaoJung,
Li FuAn,
Her GuorRong
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500811
Subject(s) - capillary electrophoresis , capillary electrophoresis–mass spectrometry , mass spectrometry , electrospray ionization , chromatography , chemistry , electrospray , electrophoresis , analytical chemistry (journal)
A sheathless interface has been developed for coupling CE with electrospray IT mass spectrometer. This interface utilized a pulsed ESI source. The use of a pulsed electrospray source allows the use of a sprayer with larger orifice, and thus alleviates the problem of column clogging during conductive coating and CE analysis. A pulsed ESI source operated at 20 Hz and 20% duty cycle was found to produce the optimal signals. For better signals, the maximum ion injection time in the IT mass spectrometer has to be set to a value close to the actual spraying time (10 ms). Using a sprayer with 50 µm od, more stable and enhanced signals were obtained in comparison with continuous CE‐ESI‐MS under the same flow rate (150 nL/min). The utility of this design is demonstrated with the analysis of synthetic drugs by CE‐MS.