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Theoretical and nomenclatural considerations of capillary electrochromatography with monolithic stationary phases
Author(s) -
Végvári Ákos,
Guttman András
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500789
Subject(s) - capillary electrochromatography , electrochromatography , capillary action , realization (probability) , nanotechnology , computer science , range (aeronautics) , dispersion (optics) , high resolution , column (typography) , materials science , process engineering , geology , optics , engineering , physics , telecommunications , remote sensing , mathematics , statistics , frame (networking) , composite material
Abstract During the past decade, CEC has been one of the few novel achievements in the field of separation science attracting a wide interest. The technology progress permitted the realization of the long‐sought idea to employ an electroosmotically driven flow through the columns improving the separations in terms of both resolution and efficiency. The early practical obstacles related to the use of conventional bead‐packed columns have been solved by the introduction of continuous beds, also known as monoliths. Hitherto, various synthesis approaches have been successfully developed producing monolithic beds in situ in capillary columns, sharing similar physical structure built up of tiny particles (in the sub‐µm range) that are covalently linked together and to the capillary wall. Parallel with the practical column technology studies, the theory of electrochromatography has been continuously developed, focusing on such basic issues as EOF characterization, separation efficiency, and peak dispersion effects. This review provides a short introduction to the theory of CEC with special attention to monolithic separation beds. The paper also summarizes the latest achievements in CEC and discusses the nomenclature, EOF characteristics, and some specific advantages of monolithic column technology.