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Poly( N , N ‐dimethylacrylamide)‐grafted polyacrylamide: A self‐coating copolymer for sieving separation of native proteins by CE
Author(s) -
Zhang Jiyou,
Tran Nguyet Thuy,
Weber Jérémie,
Slim Cyrine,
Viovy JeanLouis,
Taverna Myriam
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500771
Subject(s) - copolymer , polyacrylamide , coating , adsorption , polymer chemistry , materials science , chemistry , chemical engineering , polymer , chromatography , organic chemistry , engineering
Abstract The potential of a series of newly synthesized poly( N,N ‐dimethylacrylamide) (PDMA) grafted polyacrylamide (PAM) copolymers (P(AM‐PDMA)) as a replaceable separation medium for protein analysis was studied. A comparative study with and without copolymers was performed; the separation efficiency, analysis reproducibility and protein recovery proved that the P(AM‐PDMA) copolymers were efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. Furthermore, the size‐dependent retardation of native proteins in a representative P(AM‐PDMA) copolymer was demonstrated by Ferguson analysis. The results showed that the P(AM‐PDMA) copolymers combine the good coating property of PDMA and the sieving property of PAM and could be applied as a sieving matrix for the analysis of native proteins.