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Characterization of the antioxidative activity of novel nontoxic neuropeptides by using capillary electrophoresis
Author(s) -
Vaher Merike,
Viirlaid Säde,
Ehrlich Kersti,
Mahlapuu Riina,
Jarvet Jüri,
Soomets Ursel,
Kaljurand Mihkel
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500760
Subject(s) - chemistry , capillary electrophoresis , glutathione , glycine , hydrogen peroxide , reaction rate constant , taurine , chromatography , biochemistry , amino acid , enzyme , kinetics , quantum mechanics , physics
Abstract In the present study, we have monitored the oxidation process of novel nontoxic neuropeptides and determined its rate constants, which describe the antioxidative potential of the peptides. A capillary electrophoretic method was implemented which ensures the simultaneity of analysis of reactants and products in a short time of analysis. The rate constants of oxidation of the four novel peptides, 4‐methoxy‐ L ‐tyrosinyl‐γ‐ L ‐glutamyl‐ L ‐cysteinyl‐glycine (UPF1), D ‐serinyl‐γ‐ L ‐glutamyl‐ L ‐cysteinyl‐glycine (UPF6), 4‐methoxy‐ L ‐tyrosinyl‐α‐ L ‐glutamyl‐ L ‐cysteinyl‐glycine and D ‐serinyl‐α‐ L ‐glutamyl‐ L ‐cysteinyl‐glycine, designed by us, were compared with those of oxidation of glutathione (reduced glutathione) by using capillary electrophoresis. The second‐order rate constants were similar for all peptides if the oxidation was carried out with hydrogen peroxide ( k II = 0.208 — 0.236×10 3 /M·min). The rate constants were also determined for the mixtures of peptides. When the oxidation is caused by hydroxyl radical (OH*), the γ‐glutamate containing peptides (UPF1 and UPF6) exhibited two to four times higher antioxidative activity ( k II = 4.428 and 2.152×10 3 /M·min, respectively). The results suggest that the antioxidative potential of the peptides studied is not determined by the formation of disulphide bridge alone.