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Interactions of dextran sulfates with granulocyte colony‐stimulating factor and their effects on leukemia cells
Author(s) -
Liang Aiye,
Zhou Xiaomian,
Wang Qiuyan,
Liu Xin,
Qin Jianhua,
Du Yuguang,
Wang Keyi,
Lin Bingcheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500758
Subject(s) - molecular mass , chemistry , granulocyte , dextran , binding constant , sulfation , granulocyte colony stimulating factor , cell culture , scatchard plot , biochemistry , leukemia , binding site , biology , immunology , enzyme , chemotherapy , genetics
The interactions between granulocyte colony‐stimulating factor (G‐CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G‐CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17×10 6 M −1 and 3, respectively. DS with a molecular mass of 40 kDa also had the affinity to G‐CSF and the binding constant and binding sties were 1.01×10 6 M −1 and 8, respectively. However, DS with a molecular mass of 8 kDa and the non‐sulfated saccharide, dextran, had no affinity to G‐CSF. The results indicate that the interactions between G‐CSF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G‐CSF‐binding DS on a G‐CSF‐dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G‐CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells.