Premium
Protein expression dynamics during replicative senescence of endothelial cells studied by 2‐D difference in‐gel electrophoresis
Author(s) -
Eman Michael R.,
ReganKlapisz Elsa,
Pinkse Martijn W. H.,
Koop Inge M.,
Haverkamp Johan,
Heck Albert J. R.,
Verkleij Arie J.,
Post Jan A.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500746
Subject(s) - senescence , umbilical vein , biology , microbiology and biotechnology , gel electrophoresis , endothelial stem cell , cell , human umbilical vein endothelial cell , endothelium , cytoskeleton , in vitro , biochemistry , genetics
Abstract Endothelial senescence contributes to endothelium dysfunctionality and is thereby linked to vascular aging. A dynamic proteomic study on human umbilical vein endothelial cells, isolated from three umbilical cords, was performed. The cells were cultured towards replicative senescence and whole cell lysates were subjected to 2‐D difference gel electrophoresis (DIGE). Despite the biological variability of the three independent isolations, a set of proteins was found that showed senescence‐dependent expression patterns in all isolations. We focused on those proteins that showed significant changes, with a paired analysis of variance (RM‐ANOVA) p ‐value of ≤0.05. Thirty‐five proteins were identified with LC‐Fourier transform MS, and functional annotation revealed that endothelial replicative senescence is accompanied by increased cellular stress, protein biosynthesis and reduction in DNA repair and maintenance. Nuclear integrity becomes affected and cytoskeletal structure is also changed. Such important changes in the cell infrastructure might accelerate endothelium dysfunctionality. This study provides biological information that will initiate studies to further unravel endothelial senescence and gain more knowledge about the consequences of this process in the in vivo situation.