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Contribution of cell culture additives to the two‐dimensional protein patterns of mouse macrophages
Author(s) -
Miller Ingrid,
Radwan Marta,
Strobl Birgit,
Müller Mathias,
Gemeiner Manfred
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500744
Subject(s) - transferrin , macrophage , albumin , cell culture , microbiology and biotechnology , bone marrow , gel electrophoresis , electrophoresis , cell , biology , primary culture , chemistry , immunology , biochemistry , in vitro , genetics
Low levels of fetal calf serum (FCS), used as protein supplement in cell culture medium, were traced in preparations of primary murine macrophages (bone‐marrow‐derived macrophages (BMM) and peritoneal macrophages (PM)). Main components of this common additive were mapped in 2‐DE by means of differential image gel electrophoresis and immunoblotting. Additional washing steps in cell preparation helped to decrease the levels of the four highest abundance foetal serum proteins (serum albumin (SA), α 1 ‐fetoprotein (AFP), α 1 ‐antitrypsin (α 1 AT) and transferrin (Tf)) to less than 1% of total protein. Macrophage spot pattern was recorded in parallel and showed little variation. Results presented are supposed to be of general interest for cell preparations with similar background.