Premium
Serum protein pattern during cow pregnancy: Acute‐phase proteins increase in the peripartum period
Author(s) -
Cairoli Fausto,
Battocchio Massimiliano,
Veronesi Maria Cristina,
Brambilla Daniela,
Conserva Francesca,
Eberini Ivano,
Wait Robin,
Gianazza Elisabetta
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500742
Subject(s) - haptoglobin , orosomucoid , ice calving , acute phase protein , endometritis , pregnancy , postpartum period , luteal phase , lactation , medicine , andrology , biology , endocrinology , glycoprotein , physiology , biochemistry , inflammation , genetics
Serum collected in a time‐course mode during the pregnancy of a group of heifers was analyzed by 2‐DE under various experimental conditions to optimize resolution of all protein spots. Changes in the levels of some components were detected during the last phase of pregnancy and early postpartum. These included a decrease of α 2 ‐HS‐glycoprotein, an increase of α 1 ‐antichymotrypsin and, with a much larger and more abrupt variation, of orosomucoid and haptoglobin. These findings associate the weeks preceding calving with an acute‐phase reaction. Analysis of individual animal's sera by 1‐DE demonstrated a higher level of orosomucoid in the sera of cows developing postpartum endometritis during the 2 wk after calving ( i.e. , in the course of the infection) but a lower level during the 2 wk before calving. This observation could represent an important tool for the prepartum detection of animals prone to develop postpartum endometritis and lead to a more accurate peripartum management of those animals.