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A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis
Author(s) -
Wang Wei,
Vignani Rita,
Scali Monica,
Cresti Mauro
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500722
Subject(s) - protocol (science) , extraction (chemistry) , chromatography , computational biology , protein purification , chemistry , biology , biochemistry , medicine , pathology , alternative medicine
A simple and universally applicable protocol for extracting high‐quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.