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Parallel analysis of biomolecules on a microfabricated capillary array chip
Author(s) -
Shen Zheng,
Liu Xiaojun,
Long Zhicheng,
Liu Dayu,
Ye Nannan,
Qin Jianhua,
Dai Zhongpeng,
Lin Bingcheng
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500689
Subject(s) - biomolecule , capillary electrophoresis , capillary action , lab on a chip , nanotechnology , chip , materials science , chemistry , microfluidics , chromatography , computer science , telecommunications , composite material
This paper focused on a self‐developed microfluidic array system with microfabricated capillary array electrophoresis (μ‐CAE) chip for parallel chip electrophoresis of biomolecules. The microfluidic array layout consists of two common reservoirs coupled to four separation channels connected to sample injection channel on the soda‐lime glass substrate. The excitation scheme for distributing a 20 mW laser beam to separation channels in an array is achieved. Under the control of program, the sample injection and separation in multichannel can be achieved through six high‐voltage modules' output. A CCD camera was used to monitor electrophoretic separations simultaneously in four channels with LIF detection, and the electropherograms can be plotted directly without reconstruction by additional software. Parallel multichannel electrophoresis of series biomolecules including amino acids, proteins, and nucleic acids was performed on this system and the results showed fine reproducibility.