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Flow‐through partial‐filling affinity capillary electrophoresis using a crossreactive antibody for enantiomeric separations
Author(s) -
Grubor Nenad M.,
Armstrong Daniel W.,
Jankowiak Ryszard
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500660
Subject(s) - capillary electrophoresis , chromatography , enantiomer , chemistry , capillary action , flow (mathematics) , electrophoresis , capillary electrochromatography , thermodynamics , mechanics , physics , stereochemistry
It is demonstrated that the separation of diastereoisomers and enantiomers can be accomplished by the flow‐through partial‐filling affinity CE using a crossreactive mAb. This approach revealed differences in the binding strength of the (±)‐ cis ‐ and (±)‐ trans ‐benzo[ a ]pyrene tetrols with the anti ‐polycyclic aromatic hydrocarbon mAb and demonstrated that (+)‐enantiomers are more strongly immunocomplexed than their (−)‐counterparts. It is proposed that crossreactive monoclonal antibodies ( i.e. mAb raised against achiral molecule and possessing limited selectivity) could be effectively utilized for specific stereoisomeric differentiation and chiral separations.