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Metallomesogenic stationary phase for open‐tubular capillary electrochromatography
Author(s) -
Chen JianLian
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500628
Subject(s) - capillary electrochromatography , chemistry , siloxane , phase (matter) , polymer , dielectric , aqueous solution , ionic bonding , polar , electrophoresis , electrochromatography , ionic strength , chromatography , partition coefficient , analytical chemistry (journal) , capillary electrophoresis , materials science , organic chemistry , ion , physics , optoelectronics , astronomy
A synthetic coppermesogenic polymer is prepared and then covalently bonded to the siloxane‐based deactivated column as the stationary phases of open‐tubular CEC with essentially high phase ratio. The EOF generated from the modified phase is surveyed through conventional aqueous buffers and hydroorganic mobile phases. Zeta potentials, which are computed from the EOF data and the ratio of dielectric constant to viscosity, are plotted as a function of pH, ionic molarity, and compositional range. These plots responsible for the electroosmotic characteristic of the bonded phases are found to be like those of bare fused‐silica or deactivated columns through decreasing or increasing the ACN content in the mobile phase, respectively. This two‐phase characteristic is basically derived from the polymeric configuration with carboxylato ligands attached onto the polysiloxane backbone. Phthalates and amino acids are suitable probes to examine the two phenomena, more‐polar and less‐polar mediums, respectively, and to judge whether the chromatographic retention is the major source of separation mechanism. With the mixing modes of Lewis acid–base interaction, dispersive force, and shape discrimination, the chromatographic partition adequately accomplishes the uneasily resolved separations by only CZE mode, although the electrophoretic migration is truly somewhat involved.