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Monolithic silica capillary column with coated cellulose tris(3,5‐dimethylphenylcarbamate) for capillary electrochromatographic separation of enantiomers
Author(s) -
Qin Feng,
Xie Chuanhui,
Feng Shun,
Ou Junjie,
Kong Liang,
Ye Mingliang,
Zou Hanfa
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500622
Subject(s) - capillary electrochromatography , enantiomer , van deemter equation , capillary action , cellulose , chromatography , electrochromatography , tris , chemistry , coating , analytical chemistry (journal) , silica gel , materials science , high performance liquid chromatography , capillary electrophoresis , composite material , organic chemistry , biochemistry
Monolithic silica capillary columns were prepared by a sol‐gel process in fused‐silica capillaries with an inner diameter of 50 μm and were modified by coating of cellulose tris(3,5‐dimethylphenylcarbamate). Influences of the factors in the modification process on enantiomer separations were investigated. The prepared columns were used to perform enantiomer separations by CEC. Fifteen and two pairs of enantiomers were separated under aqueous and nonaqueous mobile phases, respectively, and most of them were baseline‐separated with very high column efficiencies. The Van Deemter curve was found flat under high linear velocity of the mobile phase, which indicated favorable kinetic properties of the prepared columns. Baseline separation of a pair of enantiomers was achieved in 90 s with high‐column efficiency by short‐end separation under high voltage.