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Experimental study of the formation of high‐resistivity zones at the gel/buffer interface in CE
Author(s) -
Khozikov Vyacheslav,
Kosobokova Olga,
Citver Gregory,
Tyshko George,
Gavrilov Dmitri N.,
Gudkov George,
Gorfinkel Vera
Publication year - 2007
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500599
Subject(s) - resistive touchscreen , joule heating , electrical resistivity and conductivity , buffer (optical fiber) , capillary action , materials science , capillary electrophoresis , joule (programming language) , interface (matter) , characterization (materials science) , polymer , analytical chemistry (journal) , biological system , mechanics , computer science , composite material , nanotechnology , chemistry , chromatography , capillary number , electrical engineering , thermodynamics , physics , power (physics) , telecommunications , biology , computer vision , engineering
A novel, nondamaging method for experimental characterization of the formation and propagation of high‐resistivity zones in CE, based on the measurement of time‐dependent Joule heating on the outer capillary surface is proposed. The method detects propagation of resistive regions in capillaries in real time and allows the estimation of their velocity and resistance. The presented experimental data are in agreement with the results of the computer simulation as well as with previous data on the subject. The proposed method is useful for the development of new polymers as well as for the refinement and optimization of new CE protocols.