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Capillary electrochromatography of biologically relevant flavonoids
Author(s) -
Stöggl Wolfgang M.,
Huck Christian W.,
Stecher Günther,
Bonn Günther K.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500540
Subject(s) - capillary electrochromatography , chemistry , chromatography , hesperetin , resolution (logic) , high performance liquid chromatography , myricetin , analyte , capillary electrophoresis , analytical chemistry (journal) , quercetin , flavonoid , kaempferol , organic chemistry , artificial intelligence , computer science , antioxidant
Flavonoids were separated utilizing CEC technique. Baseline separation of biologically relevant flavonoids was obtained using a 100 μm ID fused‐silica capillary filled with 3 μm Silica‐C 18 material and an optimized mobile phase comprising of 20 mM Tris‐HCl (pH 6.5), ACN and water at a ratio of 10/40/50 v/v/v. Separations were carried out at 25 kV and a column temperature of 25°C. The influence of relevant parameters for the CEC separation, such as buffer concentration, pH, separation voltage, and ACN concentration, was investigated and optimized. Dependencies of the electroendoosmotic flow (EOF) on these parameters and effects on the resolution of the analytes were studied. During analyses the solvents used for dissolving the samples turned out to have significant effects on the separation of flavonoids. The optimized system was then successfully used for the separation of the flavonoids epicatechin, myricetin, quercetin, naringenin, and hesperetin. CEC turned out to be a useful complementary tool for the economic analysis of flavonoids in addition to common HPLC, μHPLC, and CE methodologies. This method can be used for real applications in phytomics.