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CE‐LIF coupled with flow cytometry for high‐throughput quantitation of fluorophores in single intact cells
Author(s) -
Xiao Hua,
Li Xin,
Zou Hanfa,
Yang Ling,
Wang Yulin,
Wang Hailin,
Le X. Chris
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500536
Subject(s) - flow cytometry , chemistry , k562 cells , membrane , chromatography , high throughput screening , throughput , membrane permeability , cytometry , biophysics , cell , microbiology and biotechnology , biochemistry , biology , telecommunications , computer science , wireless
We report a method of coupled CE‐LIF detection with flow cytometry for high‐throughput determination and quantitation of fluorophores in single intact K562/S (KS) cells. The membrane properties of KS cell including fluophore transport rate and apparent permeability coefficient were further quantitatively characterized. The method has advantages for accurate quantitation and unique capacity of high‐throughput analysis. The strategy will be useful for the quantitation of fluorophores in the intact cells, such as measurement of multidrug resistance, quantitation of specific protein expression, and quantitative characterization of protein and enzyme functions.