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Recent advances in peptide and peptidomimetic stereoisomer separations by capillary electromigration techniques
Author(s) -
Scriba Gerhard K. E.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500446
Subject(s) - peptidomimetic , peptide , electromigration , chemistry , electrochromatography , capillary electrochromatography , combinatorial chemistry , nanotechnology , capillary electrophoresis , chromatography , materials science , biochemistry , composite material
As the stereochemistry of peptides determines their physicochemical properties and biological activities, analytical methods able to discriminate between peptide stereoisomers are important especially with regard to pharmaceutical peptides and peptidomimetics. The present review summarizes recent developments in peptide and peptidomimetic stereoisomer separations by capillary electromigration techniques. The majority of separations were performed by CE while only few reports have been published on the subject of electrochromatography. In addition to systematic studies on the applicability of certain buffer additives and the evaluation of specific experimental conditions, there have been attempts to understand the mechanistic aspects of peptide stereoisomer separations as well as to analyze the structure of peptide–CD complexes.